Studies on Sedative Effect of Relazovac™

  LIFEnhance‘s contract laboratories:

  Dr. D. Yang and the Research Team

 University of Texas, MD Anderson Cancer Center

 Zihong He, Chinese and Western Medicine Institute of Hubei Traditional Chinese Medicine Academy

Abstract:

6g, 3g, and 1.5g/kg (weight) of Relazovac™, equivalent to 40g, 20g, and 10g of crude drugs, which was respectively 17, 8.5, and 4 times of the dosage for adults, was given (ig) to mice for 14 days.  As a result, the number of mice that fell asleep was increased in the sub-threshold dose of pentobarbital sodium (30mg/kg).  The percentage of sleeping is 42.86, 35.71 and 7.14% respectively while that of the control group was zero.  The sleeping span of the mice in the threshold dose of sodium amytal was prolonged and the incubation period was shortened.  It is proven that the compound has the inhibitory effect on the central nervous system.

Materials:

1)    Relazovac™ extract was provided by LIFEnhance, Inc., with batch number 20020403.  0.15g extract was equivalent to 1 g crude drugs.  As required by the experiment, the extract was mixed into various concentrations and stored in refrigerator at 4C for use.

2)   Pentobarbital sodium was provided by Shanghai Branch of Chemical Reagent Packing, with batch number 940612.

3)   Sodium amytal was provided by Shanghai Supplying Station of Chemical Reagent of the Medicine Company of China , with batch number 970213.

4)   Estazolam was provided by AiBaike Medicine Industry, with batch number 20010402.

5)    Diazepam was provided by Shanghai Jiu Fu Medicine Industry, with batch number 000201.

The Animals, provided by Hubei Animal Experiment Center , were small kunming-breed mice with the weight of 20±2 g and with the same number of ♀ (male) ♂ (female).  The certificate of inspection was 19-007.  The mouse food was purchased from the same center.

Methods and Results:

 1.      The effect of Relazovac™on the sub-threshold dose of pentobarbital sodium.

 According to the literature ^[1], the mice with the weight of 18-22g and the same number of ♀♂, were divided into five groups at random, each with 14 mice: the control group, Diazepam positive group and groups of Relazovac™ with large, medium, and small dosages.  The mice were respectively given (ig) NS 20ml/kg, 0.5mg of diazepam/kg, 6g, 3g and 1.5g of Relazovac^TM/kg weight (equivalent to 40g, 20g and 10g of crude drugs, and 17.09, 8.57 and 4.27 times of the dosage for adults respectively).  The groups were given the extract (ig) once daily for 12 consecutive days.  An hour after the last dosage, pentobarbital sodium (30mg/kg) was injected into the abdominal cavity of the mice.  The righting reflex of the mice was faded for more than one minute, which was taken as the sleeping index to calculate the sleeping percentage of the mice.  The statistics were as follows (see Table1)  

Table1. The effect of Relazovac™on the sub-threshold dose of pentobarbital sodium

Groups	The number of mice	Dosage (/kg)	Percentage of sleeping	P-value
The control group	14	Ns20ml	0	0
Diazepam group	14	0.5mg	64.29	<0.01
Large dosage group	14	6g	42.86	<0.05
Medium dosage group	14	3g	35.71	<0.05
Small dosage group	14	1.5g	7.14	>0.05

 Table 1 shows the sleeping percentage of mice is increased in the sub-threshold dose of Pentobarbital sodium.  It is proven that Relazovac ^TM has the inhibitory effect on the central nervous system.

 2.   The effect of Relazovac™ on the mice’s sleeping time of sodium amytal.

 According to the literature ^[2], the mice, half of whom were male with the weight of 18-22g were divided into groups at random, each with 12 mice.  That is, the control group, Estazolam group and groups of Relazovac™ with large, medium and small dosages.  They were respectively given (ig) once a day NS 20ml/kg, 0.5mg/kg of Estazolam, and 6g, 3g and 1.5g/kg of Relazovac™ continuously for 14 days.  An hour after the last dosage, sodium amytal (50mg/kg) was injected into the abdominal cavity of the mice.  The time of falling asleep was from the fade of the righting reflex and the time from the fade of the righting reflex to waking was the sleep span.  The differences among the groups are shown in Table 2.

Table2. The effect of Relazovac™ on the mice’s sleeping of sodium amytal

Groups	The number	Dosage (/kg)	The time of falling asleep 又±SD (min)	Sleeping span 又+SD (min)
The control	12	—	7.08±3.40	20.67±7.35
Estazolam	12	0.5mg	4.00±0.95**	42.83±16.78
Large dosage	12	6g	4.17±1.99*	31.67±15.04*
Medium dosage	12	3g	3.83±1.03**	31.42±11.55*
Small dosage	12	1.5g	4.42±1.16*	30.42±14.9

n=12  compared with the control group * P<0.05 , ** P<0.01, ***P<0.001

The results show that the extract can prolong the sleeping span in the threshold dose of sodium amytal and the incubation period is shortened.  It is proven that Relazovac™ has the inhibitory effect on the central nervous system.

References:

[1] Chen QI. Research Methodology of Pharmacology of Traditional Chinese Medicine〔M), People’s Heath Press P.672.

[2] Li yikui. Experimental Methodology of Pharmacology of Traditional Chinese Medicine〔M〕Shanghai: Shanghai Science and Technology Press, 1991: 335.